Evaluation of Cytotoxicity and Genotoxicity of Insecticide Carbaryl to Flounder Gill Cells and Its Teratogenicity to Zebrafish Embryos

In this study, we determined the cytotoxicity and genotoxicity of carbamate insecticide carbaryl to flounder gill(FG) cells and its teratogenicity to zebrafish embryos. The cytotoxicity of carbaryl to FG cells was determined with methods including MTT and neutral red uptaking(NRU), lactate dehydrogenase(LDH) releasing and Hoechst 33342 and propidium idodide(PI) double staining. Moderate cytotoxicity in a concentration-dependent manner was observed. The 24 h-IC50 value of 53.48 ± 1.21, 59.13 ± 1.19 and 46.21 ± 1.24 mg L-1 carbaryl was obtained through MTT, NRU and LDH assays, respectively. Double fluorescence staining demonstrated that carbaryl induced the death of FG cells mainly through necrosis. There was no significant genotoxicity found in the FG cells exposed to the highest testing concentration of carbaryl(20 mg L-1, P 0.05) as was demonstrated by Comet assay. Zebrafish embryos exposed to carbaryl at concentrations ≥10 mg L-1 displayed moderate toxic effects on the survival, spontaneous movement, hatching, heart rates of the embryos and their development, which were evidenced by yolk and pericardial sac edemas, body length reduction and tail flexure in time- and concentration-dependent manners at specific stages. The 24 h-, 48 h- and 96 h-LC50 values of carbaryl to zebrafish embryos were 41.80 ± 1.10, 17.80 ± 1.04 and 14.46 ± 1.05 mg L-1, respectively. These results suggested that carbaryl is moderately toxic to FG cells cultured in vitro and zebrafish embryos, and the FG cells were similar to zebrafish embryos in their sensitivity to carbaryl as 24 h-IC50 and LC50 indicated.     

储层流动单元研究在油田老区挖潜中的应用——以吉林油田乾146区块开发为例

吉林油田乾146区块经多年开采,已经进入高含水、高采出阶段。通过储层流动单元研究,运用多参数模糊聚类方法将该区高台子油层由好至差划分为A、B、C、D、E 5类储层流动单元。查明了区内主要目的层系各含油小层的储层流动单元发育特征:40、39小层主要发育A、B类优质储层流动单元,42、41、38小层则以C、D、E类储层流动单元为主,各小层储层流动单元类型及展布与初始产能吻合度较高,表明储层流动单元类型的差异是控制本区油气分布的重要因素之一。综合油藏地质特征及油田开发动态数据,对各储层流动单元的注采渗流体系进行了深入剖析,归纳总结出乾146区块高台子油层XII砂组中剩余油分布的5条规律,据此在剩余油分布预测区部署了1口扩边井和2口老区加密井,投产后分别获得了11.2t/d、5.5t/d和8.6t/d的初始产能。     

南堡凹陷东营组高精度层序地层格架及沉积体系分析

在高分辨率三维地震、钻井以及大量地质资料研究基础上,根据层序地层界面特征,将南堡凹陷东营组划分为1个二级层序和4个三级层序。以初始洪泛面和最大洪泛面识别特征为依据,进一步将三级层序划分出体系域。通过层序地层格架特征及其发育位置分析,认为柳南次凹层序地层格架发育的主控因素为构造、物源和湖平面变化。应用层序地层学原理及南堡凹陷沉积充填背景研究,认为柳南次凹的沉积体系类型为冲积扇、扇三角洲、水下扇、重力流及湖泊沉积体系。冲积扇及水下扇体系在低位体系域中发育,高位体系域中扇三角洲体系和重力流发育,湖扩体系域中湖泊体系非常发育。     

滇东地区煤田构造格局与煤系赋存规律的研究

滇东地区位于特提斯和环太平洋构造体系的交接部位,煤田构造格局的总体特征是呈NNE向条带状展布。区内断层以NNE、NE向为主,受主要断裂影响,煤田在南北向呈带状,东西向呈段状展布,各煤矿区在平面上呈NE向带状分布。研究区煤系赋存受基底主干断裂的控制作用明显,变形强度由北向南逐渐增强,北部地区以褶皱构造为主,断层较少,煤层多保存于向斜构造中,保存较完整,煤层埋藏较深;南部受弥勒-师宗断裂带影响,断裂构造极为发育,对煤层破坏严重,煤系赋存不稳定。     

Geological and geochemical prediction on deeply-seated ore-bodies in Haigou gold deposit of Jilin,China

Lode 28 is the largest gold-bearing quartz vein in Haigou gold deposit,and the lode itself contains more than 30t of gold. Geochemical study shows that 12 trace elements related gold mineralization can be divided into 5 metallogenic factors(element assemblages) .The high values of F1,F2 and F4 together indicate overlapped of multiple stages of gold mineralization,revealing high potential of gold mineralization at depth;the single high value of F4 represents the root of an existing ore body while high values...     

Study on Flow Field Around Vertical Breakwater Under Different Overtopping Conditions Based on Numerical Simulation

A numerical wave flume is constructed based on the Reynolds Averaged Navier-Stokes (RANS) equations with turbulence closure by a modified k-ε model to study the viscous interactions of waves with vertical breakwaters for different overtopping cases. The governing equations,the turbulence model,boundary conditions,and solution method for the numerical wave flume are introduced briefly. The reliability of the numerical wave flume is examined by comparing the numerical results with the experimental measurement...     

Relationship between environment and the occurrence of the deep-water rose shrimp Aristeus antennatus (Risso, 1816) in the Blanes submarine canyon (NW Mediterranean)

We performed a multidisciplinary study characterizing the relationships between hydrodynamic conditions (currents and water masses) and the presence and abundance of the deep-water rose shrimp Aristeus antennatus in a submarine canyon (Blanes canyon in the NW Mediterranean Sea). This species is heavily commercially exploited and is the main target species of a bottom trawl fishery. Seasonal fluctuations in landings are attributed to spatio-temporal movements by this species associated with submarine canyons in the study area. Despite the economic importance of this species and the decreases in catches in the area in recent years, few studies have provided significant insight into the environmental conditions driving shrimp distribution. We therefore measured daily A. antennatus catches over the course of an entire year and analyzed this time series in terms of daily average temperature, salinity, mean kinetic energy (MKE), and eddy kinetic energy (EKE) values using generalized additive models and decision trees. A. antennatus was captured between 600 and 900 m in the Blanes canyon, depths that include Levantine Intermediate Water (LIW) and the underlying Western Mediterranean Deep Water (WMDW). The greatest catches were associated with relatively salty waters (38.5–38.6), low MKE values (6 and 9 cm² s⁻²) and moderate EKE values (10 and 20 cm² s⁻²). Deep-water rose shrimp occurrence appears to be driven in a non-linear manner by environmental conditions including local temperature. A. antennatus appears to prefer relatively salty (LIW) waters and low currents (MKE) with moderate variability (EKE).     

兰州地区最古老黄土剖面的粒度学及孢粉学研究

兰州地区最古老黄土剖面露头良好,地层出露较齐全,通过剖面磁性地层研究,确定兰州地区最古老黄土形成于1.84MaBP以前,对山城湾岭黄土剖面进行了粒度及孢粉的研究,探讨了第四纪沉积环境的演化。     

硬石膏制硫酸废渣联产水泥——矿产资源节约与综合利用先进适用技术

一、技术类型该技术属非金属矿综合利用技术。二、适用范围该技术适用于以天然石膏、工业副石膏、脱硫石膏等为原料,生产工业硫酸并联产水泥。三、技术内容(一)基本原理以石膏为原料生产硫酸和水泥的基本原理:以焦炭为还原剂,使CaSO4高温分解成CaO和SO2气体。CaO与掺入生料中的SiO2、Al2O3和Fe2O3反应生成水泥熟料,SO2气体随同废气由窑尾排出,经净化、洗涤、干燥、转化。     

共反射元弧叠加方法在火山岩成像中的应用

常规叠加方法假设叠加的地震道集的反射波来自地下同一个反射点,而地表观测到 的反射波场在空间上小于菲涅尔体范围是不可分辨的. 本文根据火山岩体特征,用元弧描述 火山岩的反射界面. 将来自菲涅尔体范围内的反射波沿着元弧界面求和,进而扩大叠加次数 ,达到增加反射信号能量提高地震反射波的叠加成像质量的目的. 文中给出了反射波走时参 数与地下元弧反射界面之间的联系和叠加实现的步骤. 计算结果表明,元弧叠加方法能够提 高剖面的信噪比,增加反射波同相轴的连续性.